syntaxin 16 (stx16 Search Results


syntaxin 16  (Proteintech)
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Proteintech syntaxin 16
Syntaxin 16, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
syntaxin 16 - by Bioz Stars, 2026-05
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anti-stx16  (Synaptic Systems)
90
Synaptic Systems anti-stx16
Bet1 forms novel SNARE complexes with endosomal SNAREs in MDA-MB-231 cells. (A) 3xFLAG-Bet1 interacts with STX4, Vti1b, and VAMP4 in addition to STX5. MDA-MB-231 cells stably expressing 3xFLAG-Bet1 were lysed, and the lysate was subjected to immunoprecipitation using FLAG-beads, and then the precipitate was subjected to immunoblotting for endogenous SNARE proteins. In the following blots, the brightness and contrast were adjusted so that their input bands became comparable to those of other SNAREs: STX3, STX7, <t>STX16,</t> STX17, STX18, VAMP3, VAMP7, and Vti1b. (B) An endogenous complex comprised of STX4, Bet1, Vti1b, and VAMP4 exists in MDA-MB-231 cells, but not in HeLa cells. Lysates of MDA-MB-231 and HeLa cells were subjected to immunoprecipitation of STX4, and then the co-precipitation of Bet1, Vti1b, and VAMP4 was analyzed. In the following blots, the brightness and contrast were adjusted so that their input bands became comparable to those of other SNAREs: Bet1 and VAMP4 in MDA-MB-231 cells and Bet1, VAMP4, and VAMP8 in HeLa cells. (C and D) Bet1-interacting SNAREs are colocalized with Bet1-GFP in endomembrane compartments. MDA-MB-231 cells stably expressing Bet1-GFP were transfected with FLAG-SNAREs and then subjected to immunofluorescence and confocal microscopy (C). Their colocalization was assessed by using the Manders’ overlap coefficient (D). (E–G) Depletion of STX4 and Vti1b reduces the complex of Bet1 with Vti1b and STX4, respectively. MDA-MB-231 cells stably expressing 3xFLAG-Bet1 were transfected with STX4 and Vti1b siRNAs, and then immunoprecipitation and immunoblotting (E) were performed as in A. Co-precipitated endogenous STX4 (F) and Vti1b (G) were quantified. Scale bar: 10 μm in a regular image; 2 μm in an inset. **, P < 0.01; vs. STX4 in D; vs. FLAG (Bet1), mock in F and G.
Anti Stx16, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-stx16/product/Synaptic Systems
Average 90 stars, based on 1 article reviews
anti-stx16 - by Bioz Stars, 2026-05
90/100 stars
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syntaxin 16 stx16 cytosection  (OriGene)
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Transient overexpression of STX16 NM 003763 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 5 slides per pack
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syntaxin 16 stx16 human qpcr primer pair  (OriGene)
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qSTAR qPCR primer pairs against Homo sapiens gene STX16
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syntaxin 16 stx16 nm 001134772 human tagged orf clone  (OriGene)
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STX16 Myc DDK tagged Human syntaxin 16 STX16 transcript variant 3
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syntaxin 16 stx16 human shrna lentiviral particle  (OriGene)
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STX16 Human shRNA lentiviral particles 4 unique 29mer target specific shRNA 1 scramble control 0 5 ml each 10 7 TU ml
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syntaxin 16 (stx16) (nm_001001433) human over-expression lysate  (Boster Bio)
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Transient overexpression lysate of syntaxin 16 (STX16)
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syntaxin 16 stx16 nm 001001433 human tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles STX16 Myc DDK tagged Human syntaxin 16 STX16 transcript variant 1 200ul 10 7 TU mL
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syntaxin 16 stx16 human sirna oligo duplex  (OriGene)
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STX16 Human 3 unique 27mer siRNA duplexes 2 nmol each
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syntaxin 16 stx16 nm 001134772 human untagged clone  (OriGene)
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STX16 untagged Human syntaxin 16 STX16 transcript variant 3
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Image Search Results


Bet1 forms novel SNARE complexes with endosomal SNAREs in MDA-MB-231 cells. (A) 3xFLAG-Bet1 interacts with STX4, Vti1b, and VAMP4 in addition to STX5. MDA-MB-231 cells stably expressing 3xFLAG-Bet1 were lysed, and the lysate was subjected to immunoprecipitation using FLAG-beads, and then the precipitate was subjected to immunoblotting for endogenous SNARE proteins. In the following blots, the brightness and contrast were adjusted so that their input bands became comparable to those of other SNAREs: STX3, STX7, STX16, STX17, STX18, VAMP3, VAMP7, and Vti1b. (B) An endogenous complex comprised of STX4, Bet1, Vti1b, and VAMP4 exists in MDA-MB-231 cells, but not in HeLa cells. Lysates of MDA-MB-231 and HeLa cells were subjected to immunoprecipitation of STX4, and then the co-precipitation of Bet1, Vti1b, and VAMP4 was analyzed. In the following blots, the brightness and contrast were adjusted so that their input bands became comparable to those of other SNAREs: Bet1 and VAMP4 in MDA-MB-231 cells and Bet1, VAMP4, and VAMP8 in HeLa cells. (C and D) Bet1-interacting SNAREs are colocalized with Bet1-GFP in endomembrane compartments. MDA-MB-231 cells stably expressing Bet1-GFP were transfected with FLAG-SNAREs and then subjected to immunofluorescence and confocal microscopy (C). Their colocalization was assessed by using the Manders’ overlap coefficient (D). (E–G) Depletion of STX4 and Vti1b reduces the complex of Bet1 with Vti1b and STX4, respectively. MDA-MB-231 cells stably expressing 3xFLAG-Bet1 were transfected with STX4 and Vti1b siRNAs, and then immunoprecipitation and immunoblotting (E) were performed as in A. Co-precipitated endogenous STX4 (F) and Vti1b (G) were quantified. Scale bar: 10 μm in a regular image; 2 μm in an inset. **, P < 0.01; vs. STX4 in D; vs. FLAG (Bet1), mock in F and G.

Journal: The Journal of Cell Biology

Article Title: MT1-MMP recruits the ER-Golgi SNARE Bet1 for efficient MT1-MMP transport to the plasma membrane

doi: 10.1083/jcb.201808149

Figure Lengend Snippet: Bet1 forms novel SNARE complexes with endosomal SNAREs in MDA-MB-231 cells. (A) 3xFLAG-Bet1 interacts with STX4, Vti1b, and VAMP4 in addition to STX5. MDA-MB-231 cells stably expressing 3xFLAG-Bet1 were lysed, and the lysate was subjected to immunoprecipitation using FLAG-beads, and then the precipitate was subjected to immunoblotting for endogenous SNARE proteins. In the following blots, the brightness and contrast were adjusted so that their input bands became comparable to those of other SNAREs: STX3, STX7, STX16, STX17, STX18, VAMP3, VAMP7, and Vti1b. (B) An endogenous complex comprised of STX4, Bet1, Vti1b, and VAMP4 exists in MDA-MB-231 cells, but not in HeLa cells. Lysates of MDA-MB-231 and HeLa cells were subjected to immunoprecipitation of STX4, and then the co-precipitation of Bet1, Vti1b, and VAMP4 was analyzed. In the following blots, the brightness and contrast were adjusted so that their input bands became comparable to those of other SNAREs: Bet1 and VAMP4 in MDA-MB-231 cells and Bet1, VAMP4, and VAMP8 in HeLa cells. (C and D) Bet1-interacting SNAREs are colocalized with Bet1-GFP in endomembrane compartments. MDA-MB-231 cells stably expressing Bet1-GFP were transfected with FLAG-SNAREs and then subjected to immunofluorescence and confocal microscopy (C). Their colocalization was assessed by using the Manders’ overlap coefficient (D). (E–G) Depletion of STX4 and Vti1b reduces the complex of Bet1 with Vti1b and STX4, respectively. MDA-MB-231 cells stably expressing 3xFLAG-Bet1 were transfected with STX4 and Vti1b siRNAs, and then immunoprecipitation and immunoblotting (E) were performed as in A. Co-precipitated endogenous STX4 (F) and Vti1b (G) were quantified. Scale bar: 10 μm in a regular image; 2 μm in an inset. **, P < 0.01; vs. STX4 in D; vs. FLAG (Bet1), mock in F and G.

Article Snippet: The commercial rabbit polyclonal antibodies used were anti-FLAG (Sigma-Aldrich), anti–MT1-MMP (EMD Millipore), anti-STX16 (Synaptic Systems), anti-GFP (Thermo Fisher Scientific), and anti-GPP130 (Covance).

Techniques: Stable Transfection, Expressing, Immunoprecipitation, Western Blot, Transfection, Immunofluorescence, Confocal Microscopy